Aims: This study was prompted to investigate the intestinal localization and colonization of orally administered Escherichia coli Nissle 1917 (EcN) in piglets. Methods and Results: EcN was fed to ten EcN-negative piglets (3 months) over seven consecutive days. Faecal samples were collected repeatedly and tested for EcN-DNA by a combined culture
As an intervention for dysbiosis-associated distress, the probiotic strain Escherichia coli Nissle 1917 (EcN) was evaluated for its effects on streptomycin-induced behavioral changes in mice. EcN supplementation persistently ameliorated anxiety responses in mice with streptomycin-induced dysbiosis. As an outcome of anxiety, body weight changes
EcN grows very well at the normal as well as at stressful conditions and exhibits high level of transformation efficiency and greater ability to retain heterogenous plasmid, and is highly resistant to P1 phage infection. Escherichia coli Nissle 1917 (EcN) is one of the probiotics that has drawn more attention from researchers in recent days as it extends many host beneficial effects. EcN is
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- Ψопе иηехի
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- И лечоմ ቯ φ
- ԵՒфեсвዑкегл ыни
- Եне ዬуրилችπ ፖլሴм щаጻቄβ
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The probiotic Escherichia coli strain Nissle 1917 (EcN) has been used for decades in human medicine in Central Europe for the treatment and prevention of intestinal disorders and diseases. However, the molecular mechanisms underlying its beneficial effects are only partially understood. To identify …
Oral administration of the probiotic bacterium Escherichia coli Nissle 1917 improves chronic inflammatory bowel diseases, but the molecular basis for this therapeutic efficacy is unknown. E. coli Nissle 1917 harbors a cluster of genes coding for the biosynthesis of hybrid nonribosomal peptide-polyke …
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Escherichia coli Nissle 1917 (EcN) is a probiotic strain with proven efficacy in inducing and maintaining remission of ulcerative colitis. However, the microbial factors that mediate these beneficial effects are not fully known. Gram-negative bacteria release outer membrane vesicles (OMVs) as a direct pathway for delivering selected bacterial proteins and active compounds to the host. In fact
The probiotic Escherichia coli strain Nissle 1917 (DSM 6601, Mutaflor), generally considered beneficial and safe, has been used for a century to treat various intestinal diseases. However, Nissle 1917 hosts in its genome the pks pathogenicity island that codes for the biosynthesis of the genotoxin colibactin. Colibactin is a potent DNA
Escherichia coli Nissle 1917 (EcN) is a probiotic bacterium, commonly employed to treat certain gastrointestinal disorders. It is fast emerging as an important target for the development of therapeutic engineered bacteria, benefiting from the wealth of knowledge of E. coli biology and ease of manipulation. As a result, traditional probiotic bacterial strains such as E. coli Nissle 1917 and lactic acid bacteria are the typical choice for engineering and deployment into the gut. E. As strong and significant inhibitory effects on adherent-invasive E. coli adhesion and invasion were observed in co-infection and pre-infection experiments, E. coli Nissle 1917 could be efficient for preventive or curative probiotic therapy in patients with Crohn's disease. CCK-8 assay, Hoechst 33258 staining and flow cytometry analysis were used for examining cytotoxicity of tumoricidal protein azurin toward CT26 cells. An engineered probiotic Ep-AH harboring azurin and hlpA genes was developed using Escherichia coli Nissle 1917 (EcN) chassis. Antitumor effects of Ep-AH were evaluated in the azoxymethane (AOM Increased numbers of adherent invasive Escherichia coli (AIEC) have been found in Crohn's disease (CD) patients. In this report, we investigate the potential of the probiotic Escherichia coli Nissle 1917 (EcN) to reduce features associated with AIEC pathogenicity in an already established infection …Escherichia coli Nissle 1917 (EcN) was engineered as a β-alanine producing cell factory and facilitates production of β-Alanine via fermentation, showing the potential of EcN as a valuable industrial platform. Abstract β-alanine has been used in food and pharmaceutical industries. Although Escherichia coli Nissle 1917 (EcN) is generally considered safe and engineered as living therapeuticsEscherichia coli Nissle 1917 (EcN) is a Gram‐negative probiotic, originally isolated by Dr. Alfred Nissle during World War I (Nissle, 1918; Nissle, 1925 ). EcN is serum‐sensitive and does not produce any enterotoxins or cytotoxins associated with pathogenic E. coli strains (Sonnenborn and Schulze, 2009 ). Double-blinded, placebo-controlled trials of probiotic E. coli suggest that these strains are poor colonizers of the human gut even when exposure levels are high. For example, E. coli Nissle 1917 (Mutaflor®), was detected in only 2 of 12 volunteers tested even though they orally ingested 10 9-10 10 CFU twice daily . Studies have shown that EcN may be therapeutically effective in a variety of conditions such as ulcerative colitis, diarrhoea and chronic constipation. The E. coli strain Nissle 1917 is characterised by a number of features that set it aside from the rest of its family members. As a potent probiotic, it can work to promote digestive health. .
